3^rd International Congress on Traditional & Natural Medicine October 23-24, 2019 Panorama Hotel, Prague, Czech Republic,

Prashant Pawar is an Interventional Cardiologist. He has done his research work in PVI for Paroxysmal Atrial Fibrillation. He has worked with Dr. Yash Lokhandwala the pioneer in EP study in India. He has many publications in his name. Present study was to evaluate PVI by conventional RF ablation.

Introduction: There is no Indian data on the outcome following conventional radiofrequency catheter ablation (RFA) for patients with paroxysmal atrial fibrillation (PAF). Inspite of recent advancements in this field including sophisticated three dimensional (3D) based imaging and advanced ablation catheters with contact force technology, many Indian patients will not afford such an expensive therapeutic procedure. Here in this article we have summarized the immediate and long term outcome following RFA of Indian patients with PAF using pulmonary vein (PV) electrogram based mapping and using 8 mm tip ablation catheter.

 

Methods: Fourty two consecutive patients who underwent RFA for symptomatic PAF not controlled with atleast one antiarrhythmic drugs were studied in a tertiary care institute from March 2015 to December 2018. PVI was performed solely by conventional electrophysiology procedure using 8 mm tip RF catheter and PV potential electrogram based mapping. 3D mapping was not used. Only PVI was performed. Substrate modification was not performed. Elimination of all ostial pulmonary vein potentials and complete entrance block into the pulmonary vein were considered indicative of complete electrical isolation. Follow-up visits were scheduled at four weeks, and three, six months post procedure, and every six months thereafter.

 

Results: After the procedure 34 were arrhythmia free, eight continued to have atrial fibrillation.

 

Conclusion: Conventional RFA using PV potential electrogram based mapping

and ablation with 8 mm tip catheter is safe and effective for patients with PAF with satisfactory immediate and long term outcome and negligible complications and very cost effective in our setting with limited resources.

Ibrahim Saleh Alharbi is currently working as an Internal Medicine Resident-R4 at the hospital of King Abdulaziz Medical City, Riyadh, Saudi Arabia.

Introduction: The relationship between anemia and coronary artery disease (CAD) in general population is not well defined. While some studies demonstrated increased association between iron stores and CAD as evident with increasing rate of CAD in postmenopausal women, data regarding the effect of anemia on coronary microcirculation is limited. Therefore, we examined the effect of anemia on coronary microvascular function measured by hyperemic myocardial blood flow (MBF) and non-invasive coronary flow reserve (CFR) measured by Positron Emission Tomography (PET).

 

Methods: All consecutive patients referred for clinically indicated PET between May 2011 and December 2017 who had hemoglobin (Hb) on the day of the test were included in the analysis. Patients with prior CAD, ischemia (sum difference score > 1), scar (sum stress score >3), transient ischemic dilatation and abnormal resting left ventricular function were excluded. MBF was measured by single compartment method and CFR is calculated as stress MBF/rest MBF.

 

Results: A total of 320 patients (mean age 60 ± 10 yrs, 50% female) were included of which 117 patients had Hb <11 mg/dl. There were no differences in the prevalence of hypertension or diabetes between anemic and normal Hb patients. Using spearman correlation, there was a weak, but statistically significant correlation between Hb and CFR (r=0.3589, p<0.0011) and peak MBF (r=0.1247, p=0.0258). Using multivariate linear regression, the correlations between Hb and CFR (Beta=6.03, p=<0.0001) and peak MBF (Beta=3.02, p=0.010) remained significant after adjusting for confounders.

 

Conclusions: Our analysis suggests that anemia is associated with microvascular dysfunction measured by PET derived CFR. The therapeutic implications of these findings require further testing in therapeutic studies.

Paola Vidal Rojo has completed her basic education by Cambridge University of London, has graduated from the Faculty of Medicine of the Universidad La Salle AC, and later graduated from the specialties of two of the largest Health Institutes in our country and in Latin America. She has initially in Pediatrics of Children's Hospital of Mexico, and later subspecialty in Pediatric Cardiology of the National Institute of Cardiology Ignacio Chávez, fulfilling functions of Head of Residents of the subdivision of Pediatrics. And finally she is high specialty in Medicine in Pediatric and Fetal Echocardiography in the Children's Hospital of Mexico Federico Gómez.

Mexico is a country with many inequalities, which are very notable, amongst other things, in high tech healthcare. Congenital heart diseases require in average $5,000 USD per child for a surgical treatment. Given that our average per capita income is quite low, only as few as 10% of the population can afford such healthcare, and even though 98% of the population have some sort of social security service, these are saturated, and patients may have to wait up to two years to be able to have surgery. And more so, over two million people have no access to medical services. So, we must turn to social enterprises and non-profit associations to be able to cope with this problem, and still are left with many patients without proper care. There are several areas within the country that do not have extracorporeal pump machines for open heart surgery, so they must perform surgeries without them, such as corpectomy or pulmonary banding, some of which are palliative surgeries. Many of the patients we attend arrive relatively late, considering their state. For example, we operate tetralogies of Fallot after two years of age, or anomalous pulmonary venous connections after three months. All these because of lack of planning; we have the resources and the infrastructure, but we do not yet find a way to properly manage these deficits. And so, we have so far eight nonprofit associations performing surgical procedures in congenital heart diseases throughout the country. Amongst all of them, over 1,000 children a year have been operated on, just a small percentage of all that we calculate are still awaiting surgery. Every year, more that 22,000 new children with congenital heart diseases are born, and we try to shorten the list. The first non-profit was founded in 1995, and the newest one barely this year. And all of them need mainly two things: Patients, and economical resources. We need pediatricians to redirect patients to the specialists as soon as possible and to begin medical treatment in case these referrals are not entirely feasible, and paediatric cardiologist to be familiar with all governmental, profit and nonprofit options for the patient’s benefit. We intend with this paper to share our experiences and our results in case some other countries may find it useful, as well as to urge the need for pediatric alertness, the need for resources (both human and economic), as well as an early referral, to be able to help all he children awaiting surgery to improve their quality of life.

S Pandey has obtained his Master’s degree in Yogic Science and Human Consciousness, Gurukul Mahavidyalaya College Jawalapur, Haridwar from Uttarakhand Sanskrit University, Haridwar, India. He has completed his Diploma in Bachelor of Science (ZBC) in Biology from University of Lucknow, India. He began his teaching career in Yoga in Rishikesh and in fact become one of the famous teachers of Pranayama and Philosophy in Himalaya. He teaches in famous schools like Rishikesh School of Yoga and Ayurveda, World Peace Yoga School, Himalayan Yoga Academy, Swasti Yogshala and continues his activity till nowadays. He has experience of work in foreign countries and gives many international Workshops in Russia, Poland. He has teaches in Nepal, Tibet, Thailand, Russia and many other countries.

Background: The effect of yoga practice on the depression is evaluated worldwide. We assessed the effect of yoga on the depression, human’s energy and physical bodies in our institute and observed the correlation between subjective effect of practice and charts of GDV camera (energy body and chakra system).

 

Methods: Clients of detox yoga retreat program (asana, pranayama, meditation, special detox vegetarian or vegan nutrition daily) were participated in the study. Subjective effect of yoga practice was assessed with internal questionnaire, depression symptoms were evaluated with HRDS17 Scale. The relationships between the symptoms of depression, subjective effect of yoga practice and GDV charts before and after the program were examined.

 

Results: One hundred four clients with a median age of 33, 3 years (18-73), out of 93.5% had positive subjective effect of the practice. The mean HRDS17 score was 12 (mild depression) before start of the program, after the competition of the program the mean score was four (normal). The amount of energy increased in 65% of cases; the chakra system was more align and stable in 75% after the practice.

 

Conclusion: There is a need to develop effective yoga programs for preventive care of depression and explore therapeutic effect of yoga.

Rim Frikha is an Associate Professor in Medicine and a PhD student at National School of Engineer in the University of Sfax. She is expert in Histology-Embryology and Molecular Genetic on Onco-Hematology. She has published numerous papers in reputed journals in these fields.

Introduction: High-dose methotrexate (HD-MTX) is widely used in the acute

lymphoblastic leukemia (ALL). The effect of 5, 10-methylenetetrahydrofolate

reductase (MTHFR) variants; mainly the C677T on the risk of MTX-induced

toxicity was largely investigated and enrolled in meta-analysis. However, the

results were inconsistent.

 

Aim: The aim of this study was to investigate the relationship between the C677T variant of the MTHFR gene, and the MTX-induced toxicity in Tunisian

ALL patients.

 

Materials & Methods: It was a retrospective study among 35 patients with ALL. Toxicity data was recorded after high-dose methotrexate (HD-MTX) course. Genotyping of MTHFR C677T was performed by polymerase chain reactionrestriction fragment length polymorphism (PCR- RFLP).

 

Results: Patients with ALL carrying the C677T variant were at a higher risk of

developing hepatotoxicity (RR=1.3 times).

 

Conclusion: The present result highlights the impact of MTHFR C677T variant on the MTX toxicity-induced in ALL and further studies with larger numbers of participants worldwide are required before definitive conclusions.

Pavaloiu Ramona-Daniela is currently a Post-doctoral Researcher at the Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest and also at the National Institute for Chemical-Pharmaceutical Research and Development–ICCF, Bucharest, Romania. She is a Member of 11 research projects (two as Project Manager), 14 publications (six ISI papers as First Author, five ISI papers as a Co-Author, three publications in BDI journals), one book chapter, three patents, seven patent applications, two contributions in conference proceedings, 25 communications at international and national conferences and 29 citations. Her expertise lies in innovative drug delivery systems-liposomes, nanoparticles, drug release and mathematical modeling of drug release.

Lycium barbarum (fam. Solanaceae) known as wolfberry or goji, is widely grown in the subtropical areas of the world, in Japan and Coreea, in countries from South- East of Asia and in Europe as well. In the last years, goji berries play an important role in the traditional Chinese medicine and it has attracted much attention due to their diverse biological activities, such as: liver and kidney protector and eyesight enhancer. The aim of this paper is to investigate the cytoprotective effect of an ethanolic extract of wolfberry leaves (Lycium barbarum) against H2O2 induced oxidative damage in L-929 cells. L. barbarum extract was characterized concerning its qualitative chemical composition, total phenol content and antioxidant activity by high performance liquid chromatography, folin-ciocalteu method and DPPH assay. Also, it was investigated whether pretreatment (one hour or 24 hours) with L. barbarum leaves extract had an effect on L-929 mouse fibroblasts cells cytotoxicity induced by H2O2. Data showed the presence of chlorogenic and caffeic acids and a total phenol content of 18.30 mg GAE/g dry material. DPPH method revealed significant antioxidant activity. L. barbarum extract showed a moderate cytotoxic activity. A prolonged pretreatment (24 hours) with L. barbarum extract was able to protect L-929 murine fibroblast cells against H2O2 cytotoxicity. These preliminary findings suggest that Lycium barbarum leaves can be used for the development of dietary supplements.